Genetic Analysis and Molecular Mapping of Light-Sensitive Red-Root Mutant in Rice

doi:10.1016/S1672-6308(08)60053-0

摘要/Abstract

摘要： The light-sensitive red-root mutant, designated as HG1, was newly observed from an indica rice variety, Nankinkodo, when seedlings were grown with roots exposed to natural light. The root color of the mutant began to turn slight-red when the roots were exposed to the light at the intensity of 29 μmol/(m²·s), then turned dark-red at the light intensity of 180 μmol/(m²·s), suggesting that the root color of the mutant was evidently sensitive to light. Furthermore, genetic analysis showed that the character of light-sensitive red-root of the HG1 mutant was controlled by a single dominant gene, tentatively designated as Lsr. With simple sequence repeat markers, Lsr gene was located between the markers RM252 and RM303 on chromosome 4 with the genetic distances of 9.8 cM and 6.4 cM, respectively. These results could be useful for fine mapping and cloning of Lsr gene in rice.

关键词: rice, light sensitivity, red root mutant, genetic analysis, gene mapping

Zhang Jun-zhi, Liu Xiao, Li Chao, Xiao Ke, Dong Yan-jun. Genetic Analysis and Molecular Mapping of Light-Sensitive Red-Root Mutant in Rice[J]. RICE SCIENCE, 2009, 16(1): 27-32 .

参考文献

1 Wu J, Cheng J H, Yang F C. Transcriptional regulation of anthocyanin biosynthesis in plants. Chinese J Cell Biol, 2006, 28: 453–456. (in Chinese with English abstract)
2 Tang C H. Plant Bioactive Substances. Beijing: Chemical Industry Publishing House, 2004: 218–231. (in Chinese)
3 Acquaviva R, Russo A, Galvano F, Galvano G, Barcellona M L, LiVolti G. Cyanidin and cyaniding 3-O-beta-D-glucoside as DNA cleavage protectors and antioxidants. Cell Biol Toxicol, 2003, 19(4): 243–252.
4 Lazzé M C, Pizzala R, Savio M, Stivala L A, Prosperi E, Bianchi L. Anthocyanins protect against DNA damage induced by tert-butyl-hydroperoxide in rat smooth and hepatoma cells. Mutat Res, 2003, 535(1): 103–115.
5 Russo A, La F L, Acquaviva R, Campisi A, Raciti G, Scifo C. Ochratoxin A-induced DNA damage in human fibroblast: protective effect of cyaniding 3-O-beta-D-glucoside. J Nutr Biochem, 2005, 16(1): 30–37.
6 Chung H S, Woo W S. A quinoline alkaloid with antioxidant activity from the aleurone layer of anthocyanin-pigmented rice. J Nat Prod, 2001, 64(12): 1579–1580.
7 Hu P S. Research and utilization of functional nutritional rice. China Rice, 2003(5): 3–5. (in Chinese)
8 Zhang M W, Guo B J. Summary on nutritional features and health-care function of pigmented rice grain and its processing techniques. Acta Agric Shanghai, 2002, 18(suppl): 18–24. (in Chinese with English abstract)
9 Hadagal B N, Manjunath A, Gouo V J. Linkage of genes for anthocyanin pigmentation in rice (Oryza sativa L.). Euphytica, 1980, 30: 747–754.
10 Wataru S, Taku O, Keisuke K, Chikara M, Akira S, Minoru M, Kazuhiko N, Masahiko M. The purple leaf locus of rice: The plw allele has a complex organization and includes two genes encoding basic helix-loop-helix proteins involved in anthocyanin biosynthesis. Plant Cell Physiol, 2001, 42(9): 982–991.
11 Sun M M, Han L Z, Li G X, Hong X T, Yu Y J. Advances in genetic research of gain anthocyanins content in rice. J Plant Genet Res, 2006, 7(2): 239–245. (in Chinese with English abstract)
12 Harbome J B, Grayer R J. The anthocyanins. In: Harborne J B. The Flavonoids. London: Chapman and Hall, 1988: 1–20.
13 Thakur M, Nozzolillo C. Anthocyanin pigmentation in roots of Impatiens species. Can J Bot, 1978, 56: 2898–2903.
14 Stenlid G. On the effects of some flavonoid pigments upon growth and ion absorption of wheat roots. Physiol Plant, 1961, 14: 659–670.
15 Ishikura N, Hayashi K. Anthocyanins in red roots of a radish: Studies on anthocyanins. XXXVII. Bot Mag Tokyo, 1962, 75: 28–36.
16 Duke S O, Naylor A W. Light control of anthocyanin biosynthesis in Zea seedlings. Physiol Plant, 1976, 37: 6248.
17 Solangaarachchi S M, Kevins G. Anthocyanin pigmentation in the adventitious roots of Metrosideros excelsa (Myrtaceae). New Zealand J Bot, 2001, 39: 161–166.
18 Wu S Q, Min S T, Li X S, Deng W H, Study on determination method of anthocyanin of black rice. Amino Acids & Biotic Resour, 2001, 23(4): 44–66. (in Chinese with English abstract)
19 McCouch S R, Kotcket G, Yu Z H, Wang Z Y, Khush G S, Coffman W R, Tanksley S D. Molecular mapping of rice chromosome. Theor Appl Genet, 1988, 76: 815–829.
20 Lincoln S E, Daly M J, Lander E S. Constructing genetic linkage maps with MAPMAKER/Exp 3.0. 3rd edn. Whitehead Institute Technical Report. Cambridge MA: Whitehead Institute, 1992.
21 Reddy A R. Genetic and molecular analysis of the anthocyanin pigmentation pathway in rice. In: Khush G S. Rice Genetics III. Proceedings of the Third International Rice Genetics Symposium, 16–20 Oct 1995. Manila, Philippines: International Rice Research Institute, 1996: 341–352.
22 Fan F J, Fan Y Y, Du J H, Zhuang J Y. Fine mapping of C (chromogen for anthocyanin) gene in rice. Rice Sci, 2008, 15(1): 1-6.
23 Singh A P, Prasad S C, Tomar J B, Paul A. Expressivity of pleiotropic genes governing anthocyanin pigmentation in male sterile line of rice. J Res Birsa Agric Univ, 1997, 9(2): 123–127.
24 Zhuang J Y, Yang C D, Qian H R, Zhao C Z, Zheng K L. Linkage analysis of RFLP markers and the gene for purple. Acta Genet Sin, 1996, 23(5): 372–375. (in Chinese with English abstract)
25 Phoka N, Tragonrung S, Vanavichit A. Anthocyanin intensity in rice grain is regulated by splicing efficiency of dihydroflavono1-4-reductase and is temperature sensitive. [2008-02-28]. http://dna.kps.ku.ac.th/rice_files/rice for future/ poster_rice_anthocyanin.htm.

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[11]	. [J]. Rice Science, 2021, 28(3): 217-232.
[12]	. [J]. Rice Science, 2019, 26(2): 118-124.
[13]	. [J]. Rice Science, 2019, 26(2): 77-87.
[14]	. [J]. Rice Science, 2019, 26(2): 88-97.
[15]	. [J]. Rice Science, 2019, 26(2): 98-108.